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chapterNo Access
Biomolecular Mass Spectrometry: Applications to Proteins and Peptides
- Leopold L. Ilag and
- Gianluca Maddalo
Molecules: Nucleation, Aggregation and Crystallization01 Apr 2009
Preview Abstract
Mass spectrometry is an analytical technique which, as its name implies, “weighs” molecules, and thereby can define molecular identity, interactions and reactions. With this technique, molecules from a source are ionized (i.e. made to carry a formal charge, either negative or positive) and subsequently enter the mass analyzer where ions are separated according to their mass/charge ratio (m/z), and then a spectrum is recorded as ions reach the detector. Initially applications were limited to small molecules but nowadays even large macromolecular complexes are amenable for analysis.

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