A mixed graph is a graph whose edge set consists of both oriented and unoriented edges. The Hermitian-adjacency matrix of an n-vertex mixed graph is a square matrix H(M)=[hjk] of order n, where hjk=˙ι=−hkj if there is an arc from vj to vk and hjk=1 if there is an edge between vj and vk, and hjk=0 otherwise. Let D(M)=[djj] be a diagonal matrix, where djj is the degree of vj in the underlying graph of M. The matrices L(M)=D(M)−H(M) and Q(M)=D(M)+H(M) are, respectively, the Hermitian Laplacian and Hermitian quasi-Laplacian matrix of the mixed graph M. In this paper, we first found coefficients of the characteristic polynomial of Hermitian Laplacian and Hermitian quasi-Laplacian matrices of the mixed graph M. Second, we discussed relationship between the spectra of Hermitian Laplacian and Hermitian quasi-Laplacian matrices of the mixed graph M.