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We have developed a one-day practical session to present microbiology and biotechnology to science secondary school students. For the last three years, this session has been attended by over 100 students each year, who were selected from High Secondary Schools from all over Catalonia. The feedback that we have received from the students has been extremely positive. Almost all of them described the session as a helpful experience for their preparation and the vast majority appreciated the opportunity to perform practical work in a microbiology laboratory.