During seismic action, frame columns are under reversed load. Unexpected damage of column elements may occur because of the continuous variation of axial force due to overturning moment caused by the earthquake and vertical seismic force which affect the stability of the whole structure. Therefore, the influence of the variable axial force should be considered in evaluating accurate characteristics of force and deformation of frame columns under cyclic loading. This paper presents an evaluation of hybrid simulation test method on substructure with columns under variable axial loads.