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Rotating orthogonal polarization imaging provides images of the polarization properties of scattering media which are free from surface reflections. Previously the technique has been demonstrated using manually rotated Glan–Thompson polarizers to control and analyze the polarization state of the light entering and emerging from the tissue. This paper describes a system that performs these functions using liquid crystal retarders. The system is tested using a polarizing target embedded within a scattering medium and is compared with Monte Carlo simulations. The results compare well with those obtained with manual rotation of polarizers. The liquid crystal based approach has advantages over the previous system in terms of ease of use, speed, and repeatability and is therefore an important step towards taking the technique into routine clinical use.

We developed a model to describe polarized photon scattering in biological tissues. In this model, tissues are simplified to a mixture of scatterers and surrounding medium. There are two types of scatterers in the model: solid spheres and infinitely long solid cylinders. Variables related to the scatterers include: the densities and sizes of the spheres and cylinders, the orientation and angular distribution of cylinders. Variables related to the surrounding medium include: the refractive index, absorption coefficient and birefringence. In this paper, as a development we introduce an optical activity effect to the model. By comparing experiments and Monte Carlo simulations, we analyze the backscattering Mueller matrix patterns of several tissue-like media, and summarize the different effects coming from anisotropic scattering and optical properties. In addition, we propose a possible method to extract the optical activity values for tissues. Both the experimental and simulated results show that, by analyzing the Mueller matrix patterns, the microstructure and optical properties of the medium can be obtained. The characteristic features of Mueller matrix patterns are potentially powerful tools for studying the contrast mechanisms of polarization imaging for medical diagnosis.

Polarimetry is a powerful optical tool in the biomedical field, providing more comprehensive information on the sub-wavelength micro-physical structure of a sample than traditional light intensity measurement techniques. This review summarizes the concepts and techniques of polarization and its biomedical applications. Specifically, we first briefly describe the basic principles of polarized light and the Mueller matrix (MM) decomposition method, followed by some research progress of polarimetric measurement techniques in recent years. Finally, we introduce some studies on biological tissues and cells, and then illustrate the application value of polarization optical method.