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The zoonotic illness brucellosis may spread between humans and animals in a variety of ways. The prevention and management of brucellosis depend heavily on the cleanliness of the areas around animals and the effective eradication of infection from animal housing. In our investigation, we tested a variety of disinfectants against Brucella melitensis to see how well they worked and whether they would work in various environmental settings. In addition to three different types of nano-disinfectants (Dettol with Silver-NPs, Glutaraldehyde with Silver-NPs, and Calcium oxide-NPs), our study included various conventional forms of disinfectants and antiseptics (Virkon$Ⓡ$ S, Cidex, Sodium hypochlorite, Betadine, and Dettol). For estimating the effectiveness of various types of applied disinfectants, reduction rate was employed. The findings indicated that the concentration and length of exposure time of the disinfectants employed, particularly Vircon S, had an impact on their ability to kill bacteria. However, the presence of filthy circumstances and low temperatures considerably reduced the effectiveness of disinfectants, particularly Dettol. On the other hand, nano-disinfectants, particularly glutaraldehyde containing silver nanoparticles, showed better effects than conventional ones. Our research indicated that disinfectants used in everyday life had an impact on Brucella melitensis. However, the presence of filthy environments and low temperatures reduced the bactericidal effectiveness. The impact of nano-disinfectants on Brucella was better.