Narrow Results

This study aimed to synthesize, physicochemical characterize, and evaluate the biomedical properties of silver nanoparticles (AgNPs). The methodology section included using two green methods to synthesize silver nanoparticles and comparing them to be chosen appropriately by future researchers. Two samples were obtained: one from green microwave-assisted (MSGS) called AgNPs-1 and the second sample prepared by rapid synthesis method called AgNPs-2; these samples were characterized for their physicochemical using X-ray Powder Diffraction (XRD), Dynamic Light Scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), Energy-dispersive X-ray spectroscopy (EDX). Moreover, the biomedical properties were evaluated by determining the antibacterial and cytotoxicity studies. The results observed two peaks in the UV–Vis spectroscopy were indicated at 446nm and 454nm for AgNPs-2 and AgNPs-1, respectively, which first revealed the different particle sizes and conformed in the XRD. DLS showed that the particle sizes of AgNPs-2 were 84.30nm and 121nm, respectively, while AgNPs-1 were 120.20nm and 437nm, respectively. The morphology of the synthesized samples was investigated using scanning electron microscopy (SEM) and showed different shapes, which were spherical and spherical-rod for AgNPs-2 and AgNPs-1, respectively. The presence of the silver element was indicated at 3KeV in EDX for both samples. The antibacterial test showed a significant ability to inhibit the growth of the four evaluated bacteria, and AgNPs-2 showed more effect than AgNPs-1 because of the size of the particles. The cytotoxicity study showed no toxic effect, and the average cell availability of HeLa cells was 91%. The current research demonstrated that new products from natural materials have good antibacterial properties that can be used as antibiotics with nontoxic effects in the biomedical field.