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DESIGN AND DEVELOPMENT OF A RECEPTOR ASSAY BASED ON THE INHIBITION OF CARBOXYPEPTIDASE ACTIVITY COUPLED WITH AN ELECTROCHEMICAL D-ALANINE SENSOR FOR THE DETECTION FOR β-LACTAMIC ANTIBIOTICS
Sensors and Microsystems01 Dec 2008
Preview Abstract
A microbial receptor protein with carboxypeptidase activity and a D-Alanine sensor were used to design an inhibition assay with electrochemical detectiton. This assay has been developed for the quantitative determination of β-lactam. Optimisation of the inhibition assay conditions allowed us to realise an assay with comparable analytical behaviour than the classical microbiological and other assays. The assay give a detection limit of 1 ng.mL^-1.