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Agriculture and food (agri-food) supply chains have been suffering from many issues such as lack of traceability, poor visibility and low efficiency. These issues directly and significantly affect management efforts to ensure compliance of agri-food production, improve food safety and quality, prevent food waste and reduce supply chain operation costs. This chapter will be discussing the application of blockchain technology in agri-food supply chains to address the identified issues. In this chapter, we will evaluate the potential of blockchain technology in this application area. We also point out several challenges one may face during the application process.