Authentication by DNA Sequencing of 5s rRNA and trnK Genes

The following sections are included:

• Introduction

  • Amplification and Sequences of 5s rRNA Gene Spacer Region for Identification of a Crude Drug ‘Angelica Root’, ‘Toki’ (in Japanese) and ‘Danggui’ (in Chinese)

• Materials and Methods

  • Plant Materials

  • DNA Preparation

  • Amplification of 5s rRNA Gene Spacer Region

  • Cloning and Sequencing of the PCR Products

• Results and Discussion

  • Amplification and Sequences of trnK for Identification of Crude Drugs Derived from ‘Jutsu’ (in Japanese) and ‘Zhu’ (in Chinese)

• Materials and Methods

  • Plant Materials and Crude Drugs

  • DNA Extraction (refer to Section 2.1.2)

  • PCR Amplification

  • PCR-RFLP of the PCR Product

  • Sequencing of the PCR Product

• Results and Discussion

  • Preparation of DNA from Crude Drugs

  • Identification of ‘Kara-Byaku-Jutsu’ (Dried Rhizomes of A. ovata) by PCR-RFLP of Region 1

  • Identification of ‘So-Jutsu’ (Dried Rhizome of A. lancea or A. chinensis), ‘Wa-Byaku-Jutsu’ (Dried Rhizome of A. japonica) and ‘Kara-Byaku-Jutsu’ (Dried Rhizome of A. ovata) by Direct Sequencing of Region 2

• Concluding Remarks

• Acknowledgements

• References