Authentication Based on 18S Ribosomal RNA Gene and matK Gene Sequences
The following sections are included:
Introduction
General Principles
18S ribosomal RNA gene and matK gene
PCR-RFLP and ARMS analyses
Introduction of the Medicinal Materials
Chuanxiong
Ginseng drugs
Materials and Experimental Methods
Materials
Instruments
Total DNA Preparation
PCR Amplification and Sequence Analysis of the 18S rRNA Gene
PCR-RFLP Analysis
ARMS Analysis
PCR Amplification and Sequence Analysis of the matK Gene
Results
Total DNA and PCR Product
18S rRNA Gene Sequences of ‘Chuanxiong’ and Its Related Species
18S rRNA Gene Sequences of Three Panax Species and Ginseng Drugs
PCR-RFLP Analysis on the 18S rRNA Gene of Panax Species and Ginseng Drugs
ARMS Analysis on the 18S rRNA Gene of Panax Species and Ginseng Drugs
matK Gene Sequences of Three Panax Species and Ginseng Drugs
Discussion
Acknowledgements
References
