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On NMR-based Structural Proteomics

    https://doi.org/10.1142/9789812772053_0013Cited by:0 (Source: Crossref)
    Abstract:

    The following sections are included:

    • Summary

    • Background

    • Role of NMR for Structural Proteomics

    • High-Throughput NMR Structure Production

      • Protein Expression and Preparation of 13C/15N-labeled Samples

      • Rapid NMR Data Acquisition

        • GFT Projection NMR

        • Simultaneously acquired 13C/15N resolved NOESY

        • L-optimization

        • Sparse sampling

      • (Semi-)Automated Data Analysis and Structure Calculation

      • Structure Validation

      • Benchmarking NMR Data Acquisition

      • Microgram Scale NMR-based Structural Proteomics

      • NMR Structures for Homology Modeling

    • Sructural Proteomics of Membrane Proteins

    • Perspectives

    • Acknowledgments

    • References