Extraction and purification of flavonoids from the stems of Bambusa surrecta

Flavonoids were extracted from bamboo stem via heat reflux using ethanol solvent as solvent. Extraction condition is explored experimentally in this paper, the optimal extraction conditions were as follow: alcohol/water volume ratio is 6:4, extraction time is 2 hours, extraction temperature is 80 °C, and material/solvent ratio is 1:20 (g/mL). During the purification process, cobalt ion was selected to complex with flavonoids and then disodium ethylenediamine tetraacetic acid (EDTA) was used to dissociate the cobalt-flavonoids complex. The extraction yield of flavonoids is 1.79% under the optimal extraction condition and flavonoids content can be increased to 50.8% after purification.