THE CELL EXIT OF APOPTOSIS FROM THE S-PHASE OF CELL CYCLE AND DIFFERENT CORRELATION BETWEEN MAGNESIUM AND ZINC COMPARED WITH THE OTHER KINDS OF CELLS
Abstract
The abnormal cell exit of apoptosis from S-phase, correlation between apoptosis and Mg, and between its S-phase fraction and Zn were studied in inoculated Sarcoma-180 receiving 10 or 20 Gy of 60Co gamma ray, IN VIVO, in BALB/c mice.
The apoptosis and S-phase fraction were detected by double immunostaining: 1) TUNEL that colors apoptosis brown; and 2) histone H3 mRNA probe that results in blue deposition to S-phase of cells. The concentrations of Mg and Zn were studied by PIXE.
The Mg strongly correlated with the frequency of apoptosis, but other kinds of cells did not. On the contrary, The S-phase fraction of apoptosis did not correlated with Zn, but S-phase of other kinds of cells did. This different correlation suggests the different metabolism between apoptosis and other kinds of cell.
