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Interaction of Antitumor Agent Mitoxantrone with Double Helical Synthetic Polyribonucleotides Poly(G) $\cdot$ $\cdot$ Poly(C) and Poly(I) $\cdot$ $\cdot$ Poly(C)

Department of Physics and Electrotechnics, National University of Architecture and Construction of Armenia, 105 Teryan, Yerevan 0009, Armenia

E-mail Address: babayanyura@gmail.com

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Sergey N. Hakobyan

Department of Mechanical Engineering, State Engineering University of Armenia, Teryan St. 105, Yerevan 0009, Armenia

E-mail Address: 1977poi@gmail.com

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Rusanna S. Ghazaryan

Department of Molecular Physics, Faculty of Physics, Yerevan State University, Alex Manoogian 1, Yerevan 0025, Armenia

E-mail Address: ruzkhazaryan@ysu.am

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, and

Mariam A. Shahinyan

Department of Biophysics, Faculty of Biology, Yerevan State University, Alex Manoogian 1, Yerevan 0025, Armenia

E-mail Address: m.shahinyan@ysu.am

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https://doi.org/10.1142/S1793048017500096Cited by:3 (Source: Crossref)

Abstract

The interaction of antitumor drug mitoxantrone (MTX) with double-stranded synthetic RNA homopolymers has been studied by means of spectroscopic (UV-Visible absorption, circular dichroism) techniques. The results show a base specificity in this interaction: the association constant with poly(G) $\cdot$ $\cdot$ poly(C) is higher than with poly(I) $\cdot$ $\cdot$ poly(C).

Values of changes of the system enthalpy and entropy due to complex-formation were determined through the temperature dependence of the binding constant. Calculations show that due to the intercalation interaction of MTX, the values of changes of the system entropy and enthalpy differ from those obtained at ehtidium bromide interaction with synthetic polyribonucleotides, which shows that the intercalation interaction of MTX with double-stranded RNA significantly differs from that of ethidium bromide with RNA.

Keywords:

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