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Two Types of Immunoassay Based on Nile Blue Labeling Polydopamine Nanospheres

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P. R. China

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Wenbo Lu

Key Laboratory of Magnetic Molecules & Magnetic Information Materials Ministry of Education, School of Chemistry and Material Science, Shanxi Normal University, Linfen 041004, P. R. China

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Chang Liu

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P. R. China

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Ying Wang

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P. R. China

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Jian Dong

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P. R. China

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, and

Weiping Qian

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P. R. China

E-mail Address: wqian@seu.edu.cn

Corresponding author.

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https://doi.org/10.1142/S1793292017500928Cited by:4 (Source: Crossref)

Abstract

The sandwich-type immunoassays have been developed by using electrochemical and surface-enhanced Raman scattering (SERS) techniques for the detection of carcinoembryonic antigen (CEA). Nile blue as a kind of Raman dye has been decorated on nanospheres with polydopamine resin (PDR) via $π$ -stacking interaction. The Nile blue displays the strong SERS signals as well as a characteristic electrochemical reduction peak at $-$ 0.33V (versus Ag/AgCl). The implementation of the PDR nanospheres mixing with Au nanoparticles (AuNPs/PDR) exhibits a better electrical conductivity and large SERS enhancement. The immunoassays based on Nile blue-labeled AuNPs/PDR nanospheres have been fabricated by using electrochemical and SERS techniques for the detection of CEA. The proposed immunoassay shows higher sensitivity, high selectivity, lower detection limit and long-term stability. The performances of the electrochemical immunoassay are better than that of SERS immunoassay. For the electrochemical immunoassay, the linear range occurs from 1pg/mL to 100ng/mL ( $R = 0.995$ ) with a detection limit of 0.68pg/mL and signal-to-noise ratio of 3 in the detection of CEA. The data for the analysis of human serum samples by using the electrochemical method are acceptably consistent with those obtained from the enzyme-linked immunosorbent assay (ELISA). The proposed immunoassay exhibits a promising potential for application in clinical diagnosis.

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