Special Issue on Nanobiomedicine (Part 2); Guest Editors: Feng Wang and Xiaoping ChenNo Access

Mechanism Study of Gene Delivery and Expression in PK-15 Cells Using Magnetic Iron Oxide Nanoparticles as Gene Carriers

Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, No. 12 South Street of Zhongguancun Haidian District, Beijing 100081, P. R. China

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Haixin Cui

Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, No. 12 South Street of Zhongguancun Haidian District, Beijing 100081, P. R. China

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Yongguang Yang

Department of Cancer and Cell Biology, University of Cincinnati, College of Medicine, 3125 Eden Avenue, Cincinnati, OH 45267, USA

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Xiang Zhao

Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, No. 12 South Street of Zhongguancun Haidian District, Beijing 100081, P. R. China

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Changjiao Sun

Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, No. 12 South Street of Zhongguancun Haidian District, Beijing 100081, P. R. China

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Wenjie Chen

Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, No. 12 South Street of Zhongguancun Haidian District, Beijing 100081, P. R. China

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Wei Du

Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, No. 12 South Street of Zhongguancun Haidian District, Beijing 100081, P. R. China

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, and

Jinhui Cui

Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, No. 12 South Street of Zhongguancun Haidian District, Beijing 100081, P. R. China

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https://doi.org/10.1142/S1793984414410189Cited by:10 (Source: Crossref)

Abstract

The mechanism of gene delivery and expression is one of the most important concerns raised by the development of gene delivery methods. Limited investigation is performed on how magnetic nanoparticles combine with DNA and deliver gene into mammalian cells. In this context, polyethyleneimine (PEI) coated iron oxide magnetic nanoparticles (MNPs) were used as gene carriers for binding and condensing with plasmid DNA expressing enhanced green fluorescent protein (EGFP). The morphology and structure of MNP–DNA complexes were characterized by transmission electron microscopy (TEM) and atomic force microscopy (AFM). We evidenced that large amounts of DNA wrapped around the surface of MNPs and that the MNPs were physically entrapped by the DNA arranged both horizontally and vertically. EGFP gene was successfully expressed under mediation of an external magnetic field which is necessary to efficiently target EGFP gene to the cells. Fluorescence from EGFP was separately detected in the cell cytoplasm and cell nucleus.

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