The Impact of Protein Expression Methodologies on Structural Proteomics

The following sections are included:

• Introduction

• Genetic Parameters

  • Vector Properties

  • High-throughput Cloning Strategies

    • Restriction enzyme cloning

    • Site-specific recombinational cloning

  • Ligation Independent Cloning

    • Enzyme free cloning

• Environmental Factors and Expression Strains

• Temperature and Media

  • Escherichia coli Strains

• Protein Expression Testing

• Use of Automation

• Library-based Strategies for Soluble Protein Expression

• Conclusion

• Acknowledgments

• References