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Active Compounds Isolated from Traditional Chinese Prescription Wen-Pi-Tang Protecting Against Peroxynitrite-Induced LLC-PK₁ Cell Damage

Dong Young Rhyu

Department of Medicinal Plant Resources, Mokpo National University, Jeonnam 534-729, Korea

Institute of Natural Medicine, University of Toyama, Toyama 930-0194, Japan

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Ki Sung Kang

Institute of Natural Medicine, University of Toyama, Toyama 930-0194, Japan

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Michiko Sekiya

Institute of Natural Medicine, University of Toyama, Toyama 930-0194, Japan

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Takashi Tanaka

Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8521, Japan

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Jong Cheol Park

Department of Oriental Medicine Resources, Sunchon National University, Sunchon 540-742, Korea

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, and

Takako Yokozawa

Institute of Natural Medicine, University of Toyama, Toyama 930-0194, Japan

Correspondence to: Dr. Takako Yokozawa, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan. Fax: (+81) 76-434-5068.

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https://doi.org/10.1142/S0192415X08006211Cited by:4 (Source: Crossref)

Abstract

Wen-Pi-Tang, a traditional Chinese prescription, has been widely used for the treatment of patients with moderate chronic renal failure in China. Although the protective effect of Wen-Pi-Tang on peroxynitrite (ONOO^-)-induced renal tubular epithelial LLC-PK₁ cell damage was elucidated in our previous research, the active components of Wen-Pi-Tang have not yet been fully clarified. Therefore in the present study, we investigated the active components by using a cellular ONOO^-generation system. As a result, p-coumaric acid, 4-(4'-hydroxylphenyl)-2-butanone 4'-O-glucopyranoside, gallic acid 3-O-(6'-O-galloyl)-β-d-glucopyranoside, procyanidin B-1, procyanidin B-3, and (+)-catechin were isolated as active compounds inhibiting cellular ONOO^- formation and cytotoxicity. In particular, the content of (+)-catechin was significantly higher than those of the other compounds, and the (+)-catechin structure was located in procyanidins B-1 and B-3. Therefore, the major bioactivity of Wen-Pi-Tang against ONOO^--induced cytotoxicity in LLC-PK₁ cells was thought to be mediated by (+)-catechin. Although we cannot disregard the synergetic effect of various components in Wen-Pi-Tang, (+)-catechin is a major active compound protecting against ONOO^--induced LLC-PK₁ cell damage and may be used as an index to qualify the ONOO^--inhibitory activity of Wen-Pi-Tang extract.

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References

G. E. Arteel, K. Briviba and H. Sies, FEBS Lett. 445, 226 (1999), DOI: 10.1016/S0014-5793(99)00073-3. Crossref, Medline, Web of Science, Google Scholar
J. S. Beckman and W. H. Koppenol, Am. J. Physiol. 271, 1424 (1996). Crossref, Google Scholar
H. Y. Chunget al., J. Agric. Food Chem. 46, 4484 (1998), DOI: 10.1021/jf980556u. Crossref, Web of Science, Google Scholar
C. E. Crosset al., Ann. Intern. Med. 107, 526 (1987). Crossref, Medline, Web of Science, Google Scholar
I. Y. Haddadet al., Am. J. Physiol. 267, 242 (1994). Web of Science, Google Scholar
R. E. Huie and S. Padmaja, Free Radic. Res. Commun. 18, 195 (1993), DOI: 10.3109/10715769309145868. Crossref, Medline, Google Scholar
H. Kaur, M. Whiteman and B. Halliwell, Free Radic. Res. 26, 71 (1997), DOI: 10.3109/10715769709097786. Crossref, Medline, Web of Science, Google Scholar
L. O. Klotz, P. Schroeder and H. Sies, Free Radic. Biol. Med. 33, 737 (2002), DOI: 10.1016/S0891-5849(02)00892-4. Crossref, Medline, Web of Science, Google Scholar
, Defenses against peroxynitrite: selenocompounds and flavonoids L. O. Klotz and H. Sies . Google Scholar
H. Maeda and T. Akaike, Biochemistry (Mosc.) 63, 854 (1998). Medline, Web of Science, Google Scholar
T. Mitsumaet al., J. Med. Pharm. Soc. WAKAN-YAKU 1, 266 (1984). Google Scholar
T. Mitsumaet al., Jpn. J. Nephrol. 29, 195 (1987). Medline, Web of Science, Google Scholar
T. Mitsumaet al., Jpn. J. Nephrol. 41, 769 (1999). Web of Science, Google Scholar
T. Mosmann, J. Immunol. Methods 65, 55 (1983), DOI: 10.1016/0022-1759(83)90303-4. Crossref, Medline, Web of Science, Google Scholar
G. Nonakaet al., Chem. Pharm. Bull. 29, 2862 (1981). Crossref, Web of Science, Google Scholar
G. Nonaka and I. Nishioka, Chem. Pharm. Bull. 31, 1652 (1983). Crossref, Web of Science, Google Scholar
H. Oura, P. D. Zheng and T. Yokozawa, J. Med. Pharm. Soc. WAKAN-YAKU 1, 209 (1984). Google Scholar
R. Radiet al., Free Radic. Biol. Med. 33, 1451 (2002), DOI: 10.1016/S0891-5849(02)01111-5. Crossref, Medline, Web of Science, Google Scholar
D. Y. Rhyuet al., Free Radic. Res. 36, 1261 (2002), DOI: 10.1080/1071576021000016490. Crossref, Medline, Web of Science, Google Scholar
D. Y. Rhyuet al., Am. J. Chin. Med. 35, 127 (2007). Link, Web of Science, Google Scholar
M. Sadeghipour, R. Terreux and J. Phipps, Toxicol. In Vitro 19, 155 (2005), DOI: 10.1016/j.tiv.2004.06.009. Crossref, Medline, Web of Science, Google Scholar
T. Tanaka, G. Nonaka and I. Nishioka, Phytochemistry 22, 2575 (1983), DOI: 10.1016/0031-9422(83)80168-X. Crossref, Web of Science, Google Scholar
S. Y. Tanget al., Free Radic. Biol. Med. 36, 1575 (2004), DOI: 10.1016/j.freeradbiomed.2004.03.017. Crossref, Medline, Web of Science, Google Scholar
R. C. Thuraisinghamet al., Kidney Int. 57, 1968 (2000), DOI: 10.1046/j.1523-1755.2000.00046.x. Crossref, Medline, Web of Science, Google Scholar
N. Yamabeet al., J. Pharm. Pharmacol. 57, 1637 (2005), DOI: 10.1211/jpp.57.12.0014. Crossref, Medline, Web of Science, Google Scholar
T. Yokozawaet al., J. Tradit. Med. 17, 41 (2000). Web of Science, Google Scholar
T. Yokozawa, D. Y. Rhyu and S. Owada, J. Tradit. Med. 18, 147 (2001). Web of Science, Google Scholar
T. Yokozawa, D. Y. Rhyu and E. J. Cho, J. Pharm. Pharmacol. 55, 1405 (2003), DOI: 10.1211/0022357021783. Crossref, Medline, Web of Science, Google Scholar