Abstract
The fluorescence-based in vivo flow cytometry (IVFC) is an emerging tool to monitor circulating cells in vivo. As a noninvasive and real-time diagnostic technology, the fluorescence-based IVFC allows long-term monitoring of circulating cells without changing their native biological environment. It has been applied for various biological applications (e.g., monitoring circulating tumor cells). In this work, we will review our recent works on fluorescence-based IVFC. The operation principle and typical biological applications will be introduced. In addition, the recent advances in IVFC flow cytometry based on photoacoustic effects and other label-free detection methods such as imaging-based methods, diffuse-light methods, hybrid multimodality methods and multispectral methods are also summarized.
References
- 1. , Practical Flow Cytometry, M. HowardM. D. Shapiro, (eds.), 3rd edition (Wiley-Liss, Inc, New York, NY, USA, 2010), pp. 490โ491. Google Scholar
- 2. , โPrinciples of flow cytometry,โ J. Soc. Anal. Cytol. 3(S3), 7 (1988). Google Scholar
- 3. , โFlow cytometry: Principles and clinical applications in hematology,โ Clin. Chem. 46(8), 1221โ1229 (2000). Google Scholar
- 4. , โIn vivo flow cytometer for real-time detection and quantification of circulating cells,โ Opt. Let. 29(1), 77โ79 (2004). Web of Science,ย Google Scholar
- 5. , โIn vivo flow cytometry: A horizon of opportunities,โ Cytom. A 79(10), 737โ745 (2011). Web of Science,ย Google Scholar
- 6. , โFluorescence detection, enumeration and characterization of single circulating cells in vivo: Technology, applications and future prospects,โ Phys. Med. Biol. 63(1), 01TR01 (2017). Web of Science,ย Google Scholar
- 7. , โCell labeling approaches for fluorescence-based in vivo flow cytometry,โ Cytom. A 79(10), 758โ765 (2011). Web of Science,ย Google Scholar
- 8. , โIn vivo flow cytometry: A new method for enumerating circulating cancer cells,โ Cancer Res. 64(15), 5044 (2004). Web of Science,ย Google Scholar
- 9. , โIn vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment,โ Nature 435(7044), 969 (2005). Web of Science,ย Google Scholar
- 10. , โTwo-color, double-slit in vivo flow cytometer,โ Opt. Lett. 32(20), 2993โ2995 (2007). Web of Science,ย Google Scholar
- 11. , โCirculation times of prostate cancer and hepatocellular carcinoma cells by in vivo flow cytometry,โ Cytom. A 79(10), 848โ854 (2011). Web of Science,ย Google Scholar
- 12. , โNear infrared in vivo flow cytometry for tracking fluorescent circulating cells,โ Cytom. A 87(9), 878โ884 (2015). Web of Science,ย Google Scholar
- 13. , โIn vivo imaging flow cytometer,โ Opt. Express 14(17), 7789โ7800 (2006). Web of Science,ย Google Scholar
- 14. , โA computer vision approach to rare cell in vivo fluorescence flow cytometry,โ Cytom. A 83(12), 1113โ1123 (2013). Web of Science,ย Google Scholar
- 15. , โPerformance of computer vision in vivo flow cytometry with low fluorescence contrast,โ J. Biomed. Opt. 20(13), 035005 (2015). Google Scholar
- 16. , โToward lower contrast computer vision in vivo flow cytometry,โ 36th Annual Int. Conf. IEEE Engineering in Medicine and Biology Society, pp. 4256โ4259, IEEE New York (2014). Google Scholar
- 17. , โHigh-speed microscopy for in vivo monitoring of lymph dynamics,โ J. Biophoton. 11(8), e201700126 (2017). Web of Science,ย Google Scholar
- 18. , โVisualizing interactions of circulating tumor cell and dendritic cell in the blood circulation using in vivo imaging flow cytometry,โ IEEE Trans. Biomed. Eng. 54(4), 2891068 (2019). Google Scholar
- 19. C. Wang and X. Wei โStudying liver cancer metastasis by in vivo imaging and flow cytometerโ, Proc. SPIE Vol. 7634, Optical Sensors and Biophotonics, Article ID: 76340H (25 November 2009); https://doi.org/10.1117/12.851973. Google Scholar
- 20. , โReal-time monitoring of rare circulating hepatocellular carcinoma cells in an orthotopic model by in vivo flow cytometry assesses resection on metastasis,โ Cancer Res. 72(10), 2683โ2691 (2012). Web of Science,ย Google Scholar
- 21. , โMonitoring circulating prostate cancer cells by in vivo flow cytometry assesses androgen deprivation therapy on metastasis,โ Cytom. A 93(5), 517โ524 (2018). Web of Science,ย Google Scholar
- 22. , โCharacterization of circulating tumor cell aggregates identified in patients with epithelial tumors,โ Phys. Biol. 9(1), 016001 (2012). Web of Science,ย Google Scholar
- 23. , โCirculating tumor cell clusters are oligoclonal precursors of breast cancer metastasis,โ Cell 158, 1110โ1122 (2014). Web of Science,ย Google Scholar
- 24. , โProportion of circulating tumor cell clusters increases during cancer metastasis,โ Cytom. A 91(3), 250โ253 (2016). Web of Science,ย Google Scholar
- 25. , โSystemically infused mesenchymal stem cells show different homing profiles in healthy and tumor mouse models,โ Stem Cells Trans. Med. 6(4), 1120โ1131 (2017). Web of Science,ย Google Scholar
- 26. , โNoninvasive monitoring of nanoparticle clearance and aggregation in blood circulation by in vivo flow cytometry,โ J. Control. Release 278, 66โ73 (2018). Web of Science,ย Google Scholar
- 27. , โUltra-fast photoacoustic flow cytometry with a 0.5 MHz pulse repetition rate nanosecond laser,โ Opt. Express 18(8), 8605โ8620 (2010). Web of Science,ย Google Scholar
- 28. , โIn vivo magnetic enrichment and multiplex photoacoustic detection of circulating tumour cells,โ Nat. Nanotechnol. 4(12), 855โ860 (2009). Web of Science,ย Google Scholar
- 29. , โPhotoacoustic flow cytometry: Principle and application for real-time detection of circulating single nanoparticles, pathogens, and contrast dyes in vivo,โ J. Biomed. Opt. 12(5), 051503 (2007). Web of Science,ย Google Scholar
- 30. , โNanoparticles coated with neutrophil membranes can effectively treat cancer metastasis,โ ACS Nano 11(2), 1397โ1411 (2017). Web of Science,ย Google Scholar
- 31. , โNeovasculature and circulating tumor cells dual-targeting nanoparticles for the treatment of the highly-invasive breast cancer,โ Biomaterials 113, 1โ17 (2017). Web of Science,ย Google Scholar
- 32. , โIn vivo fluorescence spectroscopy and imaging for oncological applications,โ Photochem. Photobiol. 68(5), 603โ632 (1998). Web of Science,ย Google Scholar
- 33. , โIn vivo cell characteristic extraction and identification by photoacoustic flow cytography,โ Biomed. Opt. Express 6(10), 3748โ3756 (2015). Web of Science,ย Google Scholar
- 34. , โIn vivo flow cytometry of circulating clots using negative photothermal and photoacoustic contrasts,โ Cytom. A 79(10), 814โ824 (2011). Web of Science,ย Google Scholar
- 35. , โIn vivo ultra-fast photoacoustic flow cytometry of circulating human melanoma cells using near-infrared high-pulse rate lasers,โ Cytom. A 79(10), 825โ833 (2011). Web of Science,ย Google Scholar
- 36. , โIn vivo plant flow cytometry: A first proof-of-concept,โ Cytom. A 79(10), 855โ865 (2011). Web of Science,ย Google Scholar
- 37. , โIn vivo photoacoustic flow cytometry for early malaria diagnosis,โ Cytom. A 89(6), 531โ542 (2016). Web of Science,ย Google Scholar
- 38. , โIn vivo, label-free, and noninvasive detection of melanoma metastasis by photoacoustic flow cytometry,โ Proc. SPIE 8944, 89440Q (2014). Google Scholar
- 39. , โIn vivo detection of circulating tumor cells during tumor manipulation,โ Proc. of SPIE 8565, 85652Hโ1 (2013). Google Scholar
- 40. , โReal-time monitoring of circulating tumor cell release during tumor manipulation using in vivo photoacoustic and fluorescent flow cytometry,โ Head Neck 36(8), 1207โ1215 (2014). Web of Science,ย Google Scholar
- 41. , โIn vivo label-free photoacoustic flow cytography and on-the-spot laser killing of single circulating melanoma cells,โ Sci. Rep. 6(1), 39616 (2016). Web of Science,ย Google Scholar
- 42. , โDiffuse fluorescence fiber probe for in vivo detection of circulating cells,โ J. Biomed. Opt. 22(3), 037004 (2017). Web of Science,ย Google Scholar
- 43. , โMaximum likelihood tomographic reconstruction of extremely sparse solutions in diffuse fluorescence flow cytometry,โ Opt. Lett. 38(13), 2357 (2013). Web of Science,ย Google Scholar
- 44. , โImproved diffuse fluorescence flow cytometer prototype for high sensitivity detection of rare circulating cells in vivo,โ J. Biomed. Opt. 18(7), 077002 (2013). Web of Science,ย Google Scholar
- 45. , โIn vivo confocal scanning laser microscopy of human skin: Melanin provides strong contrast,โ J. Invest. Dermatol. 104(6), 946โ952 (1995). Web of Science,ย Google Scholar
- 46. , โReflectance confocal microscopy of skin in vivo: From bench to bedside,โ Lasers Surg. Med. 49(1), 7โ19 (2016). Web of Science,ย Google Scholar
- 47. , โRetinal flow cytometer,โ Opt Lett. 32(23), 3450โ3452 (2007). Web of Science,ย Google Scholar
- 48. , โIn vivo Raman flow cytometry for real time detection of carbon nanotube kinetics in lymph, blood, and tissues,โ J. Biomed. Opt. 14(2), 021006 (2009). Web of Science,ย Google Scholar
- 49. , โNoninvasive imaging of flowing blood cells using label-free spectrally encoded flow cytometry,โ Biomed. Opt. Express 3(6), 1455โ1464 (2012). Web of Science,ย Google Scholar
- 50. , โIn vivo quantitation of rare circulating tumor cells by multiphoton intravital flow cytometry,โ Proc. Natl. Acad. Sci. 104(28), 11760โ11765 (2007). Web of Science,ย Google Scholar
- 51. , โMultiphoton flow cytometry strategies and applications,โ Cytom. A 79(10), 775โ788 (2011). Web of Science,ย Google Scholar