World Scientific
  • Search
    Quick Search in Journals
    Access type:
    Quick Search anywhere
    Access type:
    Advanced Search
  • My Cart
  •   
Skip main navigation
Close Drawer MenuOpen Drawer Menu

Cookies Notification

We use cookies on this site to enhance your user experience. By continuing to browse the site, you consent to the use of our cookies. Learn More
×

Our site uses Javascript to enchance its usability.You can disable your ad blocker or whitelist our website www.worldscientific.com to view the full content.

Select your blocker:

Adblock Plus Instructions

  1. Click the AdBlock Plus icon in the extension bar
  2. Click the blue power button
  3. Click refresh
Our website is made possible by displaying certain online content using javascript.
In order to view the full content, please disable your ad blocker or whitelist our website www.worldscientific.com.

System Upgrade on Tue, Oct 25th, 2022 at 2am (EDT)

Existing users will be able to log into the site and access content. However, E-commerce and registration of new users may not be available for up to 12 hours.
For online purchase, please visit us again. Contact us at [email protected] for any enquiries.
No Access

CELL LADEN ALGINATE/ALBUMIN HYDROGEL FIBERS FOR POTENTIAL SKIN TISSUE ENGINEERING APPLICATIONS

    https://doi.org/10.4015/S101623721850045XCited by:0 (Source: Crossref)
    Previous
    Next

    Abstract

    Alginate hydrogel fibers are receiving a great attention for tissue engineering applications. However, an important limitation of alginate is that it does not provide cell adhesion motifs. In this work, albumin was blended with alginate to improve the compatibility of alginate fibers with cells. Cell laden alginate/albumin fibers, potentially usable for skin regeneration, were obtained through a spinning process, by extruding an alginate/albumin solution containing cells into a calcium chloride solution. Cell laden pure alginate fibers were prepared for comparison. Plain alginate and alginate/albumin fibers were also produced. Morphological, mechanical and functional properties of the produced fibers were investigated. In addition, the ability of the fibers to release albumin and to support the viability and growth of A549 cells embedded into them was studied. Fibers with a uniform shape and an average diameter within the range 550–570μm were produced. The water content was 94.6±0.4% for alginate fibers, and 94.9±0.3% for alginate/albumin fibers. Stress–strain tests showed, up to a strain value of 20%, the same Young’s modulus for the produced fibers, regardless of the presence of albumin. Overall, obtained results demonstrated that morphology, size, hydrophilicity and mechanical properties were not affected by albumin. Albumin was gradually released over a period of 4 days, with a residual amount (13%) remaining into the fibers. Viability test was carried out on A549 cells, laden inside alginate and alginate/albumin fibers, to evaluate cell proliferation ability. A favorable effect of albumin on the loaded cells was evidenced by a faster kinetics of growth.

    References

    • 1. Puppi D, Zhang X, Yang L, Chiellini F, Sun X, Chiellini E , Nano/microfibrous polymeric constructs loaded with bioactive agents and designed for tissue engineering applications: A review, J Biomed Mater Res B Appl Biomater 102B :1562, 2014. Crossref, Web of Science, Google Scholar
    • 2. Chirani N, Yahia LH, Gritsch L, Motta FL, Chirani S, Fare S , History and applications of hydrogels, J Biomed Sci 4 :1, 2015. Google Scholar
    • 3. Lin HY, Peng CW, Wu WW , Fibrous hydrogel scaffolds with cells embedded in the fibers as a potential tissue scaffold for skin repair, J Mater Sci Mater Med 25 :259, 2014. Crossref, Web of Science, Google Scholar
    • 4. Lee KY, Mooney DJ , Alginate: Properties and biomedical applications, Prog Polym Sci 37 :106, 2012. Crossref, Web of Science, Google Scholar
    • 5. Lin HY, Wang HW , The influence of operating parameters on the drug release and antibacterial performances of alginate fibrous dressings prepared by wet spinning, Biomater 2 :321, 2012. Google Scholar
    • 6. Rowley JA, Madlambayan G, Mooney DJ , Alginate hydrogels as synthetic extracellular matrix materials, Biomaterials 20 :45, 1999. Crossref, Web of Science, Google Scholar
    • 7. Ellmerer M, Schaupp L, Brunner GA, Sendlhofer G, Wutte A, Wach P, Pieber TR , Measurement of interstitial albumin in human skeletal muscle and adipose tissue by open-flow microperfusion, Am J Physiol Endocrinol Metab 278 :E352, 2000. Crossref, Web of Science, Google Scholar
    • 8. Francis GL , Albumin and mammalian cell culture: Implications for biotechnology applications, Cytotechnology 62 :1, 2010. Crossref, Web of Science, Google Scholar
    • 9. Elsadek B, Kratz F , Impact of albumin on drug delivery — New applications on the horizon, J Control Release 157 :4, 2012. Crossref, Web of Science, Google Scholar
    • 10. Peters T Jr, Stewart AJ , Albumin research in the 21st century, Biochim Biophys Acta 1830 :5351, 2013. Crossref, Web of Science, Google Scholar
    • 11. Jochems CE, van der Valk JB, Stafleu FR, Baumans V , The use of fetal bovine serum: Ethical or scientific problem?, Altern Lab Anim 30 :219, 2002. Crossref, Web of Science, Google Scholar
    • 12. Selvaggi TA, Walker RE, Fleisher TA , Development of antibodies to fetal calf serum with arthus-like reactions in human immunodeficiency virus-infected patients given syngeneic lymphocyte infusions, Blood 89 :776, 1997. Crossref, Web of Science, Google Scholar
    • 13. Bernards MT, Qin C, Jiang S , MC3T3-E1 cell adhesion to hydroxyapatite with adsorbed bone sialoprotein, bone osteopontin, and bovine serum albumin, Colloids Surf B Biointerfaces 64 :236, 2008. Crossref, Web of Science, Google Scholar
    • 14. Weszl M, Skaliczki G, Cselenyák A, Kiss L, Major T, Schandl K, Bognár E, Stadler G, Peterbauer A, Csönge L, Lacza Z , Freeze-dried human serum albumin improves the adherence and proliferation of mesenchymal stem cells on mineralized human bone allografts, J Orthop Res 30 :489, 2012. Crossref, Web of Science, Google Scholar
    • 15. Horváthy DB, Vácz G, Szabó T, Szigyártó IC, Toró I, Vámos B, Hornyák I, Renner K, Klára T, Szabó BT, Dobó-Nagy C, Doros A, Lacza Z , Serum albumin coating of demineralized bone matrix results in stronger new bone formation, J Biomed Mater Res B Appl Biomater 104 :126, 2016. Crossref, Web of Science, Google Scholar
    • 16. Onoe H, Takeuchi S , Cell-laden microfibers for bottom-up tissue engineering, Drug Discov Today 20 :236, 2015. Crossref, Web of Science, Google Scholar
    • 17. Pereira RF, Sousa A, Barrias CC, Bayat A, Granja PL, Bartolo PJ , Advances in bioprinted cell-laden hydrogels for skin tissue engineering, Biomanuf Rev 2 :1, 2017. Crossref, Google Scholar
    • 18. Dunigan DD, Waters SB, Owen TC , Aqueous soluble tetrazolium/formazan MTS as an indicator of NADH- and NADPH-dependent dehydrogenase activity, Biotechniques 19 :640, 1995. Web of Science, Google Scholar
    • 19. Zwietering MH, Jongenburger I, Rombouts FM, van’t Riet K , Modeling of the bacterial growth curve, Appl Environ Microbiol 56 :1875, 1990. Crossref, Web of Science, Google Scholar
    • 20. Barnie PA, Zhang P, Lu P, Chen X, Su Z, Wang S, Xu H , CpG-oligodeoxynucleotides suppress the proliferation of A549 lung adenocarcinoma cells via toll-like receptor 9 signaling and upregulation of Runt-related transcription factor 3 expression, Biomed Rep 2 :374, 2014. Crossref, Google Scholar
    • 21. Sakai S, Yamaguchi S, Takei T, Kawakami K , Oxidized alginate-cross-linked alginate/gelatin hydrogel fibers for fabricating tubular constructs with layered smooth muscle cells and endothelial cells in collagen gels, Biomacromolecules 9 :2036, 2008. Crossref, Web of Science, Google Scholar
    • 22. Jun Y, Kim MJ, Hwang YH, Jeon EA, Kang AR, Lee SH, Lee DY , Microfluidics-generated pancreatic islet microfibers for enhanced immunoprotection, Biomaterials 34 :8122, 2013. Crossref, Web of Science, Google Scholar
    • 23. Fleischer S, Shapira A, Regev O, Nseir N, Zussman E, Dvir T , Albumin fiber scaffolds for engineering functional cardiac tissues, Biotechnol Bioeng 111 :1246, 2014. Crossref, Web of Science, Google Scholar
    • 24. ASTM F2315 - 03 Standard Guide for Immobilization or Encapsulation of Living Cells or Tissue in Alginate Gels. ASTM Book of Standards, Vol. 13, 2017. Google Scholar
    • 25. Hu X, Lu L, Xu C, Li X , Mechanically tough biomacromolecular IPN hydrogel fibers byenzymatic and ionic crosslinking, Int J Biol Macromol 72 :403, 2015. Crossref, Web of Science, Google Scholar
    • 26. Cuadros TR, Skurtys O, Aguilera JM , Mechanical properties of calcium alginate fibers produced with a microfluidic device, Carbohyd Polym 89 :1198, 2012. Crossref, Web of Science, Google Scholar
    • 27. Tibbitt MW, Anseth KS , Hydrogels as extracellular matrix mimics for 3D cell culture, Biotechnol Bioeng 103 :655, 2009. Crossref, Web of Science, Google Scholar
    • 28. Jeong KH, Park D, Lee YC , Polymer-based hydrogel scaffolds for skin tissue engineering applications: A mini-review, J Polym Res 24 :112, 2017. Crossref, Web of Science, Google Scholar
    • 29. Sun JY, Zhao X, Illeperuma WR, Chaudhuri O, Oh KH, Mooney DJ, Vlas-sak JJ, Suo Z , Highly stretchable and tough hydrogels, Nature 489 :133, 2012. Crossref, Web of Science, Google Scholar
    • 30. Neiser S, Draget KI, Smidsrød O , Interactions in bovine serum albumin–calcium alginate gel systems, Food Hydrocoll 13 :445, 1999. Crossref, Web of Science, Google Scholar
    • 31. Peng CW, Lin HY, Wang HW, Wu WW , The influence of operating parameters on the drug release and anti-bacterial performances of alginate wound dressings prepared by three-dimensional plotting, Mater Sci Eng C 32 :2491, 2012. Crossref, Web of Science, Google Scholar
    • 32. Zhao Y, Li F, Carvajal MT, Harris MT , Interactions between bovine serum albumin and alginate: An evaluation of alginate as protein carrier, J Colloid Interface Sci 332 :345, 2009. Crossref, Web of Science, Google Scholar
    • 33. Liao IC, Wan ACA, Yim EKF, Leong KW , Controlled release from fibers of polyelectrolyte complexes, J Control Release 104 :347, 2005. Crossref, Web of Science, Google Scholar
    • 34. Qi H, Hu P, Xu J, Wang A , Encapsulation of drug reservoirs in fibers by emulsion electrospinning: Morphology characterization and preliminary release assessment, Biomacromolecules 7 :2327, 2006. Crossref, Web of Science, Google Scholar
    • 35. Doebis C, Ritter T, Brandt C, Schönberger B, Volk HD, Seifert M , Efficient in vitro transduction of epithelial cells and keratinocytes with improved adenoviral gene transfer for the application in skin tissue engineering, Transpl Immunol 9 :323, 2002. Crossref, Web of Science, Google Scholar
    • 36. Vig K, Chaudhari A, Tripathi S, Dixit S, Sahu R, Pillai S, Dennis VA, Singh SR , Advances in skin regeneration using tissue engineering, Int J Mol Sci 18 :E789, 2017. Crossref, Web of Science, Google Scholar
    Remember to check out the Most Cited Articles!

    Notable Biomedical Titles
    Authors from Harvard, Rutgers University, University College London and more!