The anaerobic ammonium oxidation (anammox) microbe in an upflow anaerobic sludge blanket (UASB) bioreactor was successfully enriched under inorganic and dark condition. Seven species were isolated and purified in conventional methods from the activated sludge. Based on the morphological, physiological, biochemical and 16S rRNA analysis, these bacteria belonged to Pseudomonas sp, Clostridium beijerinckii, Bacillus cereus, two kinds of Bacillus sp., Sphingosinicella microcystinivorans and unidentified bacterium. The cells concentration of E1 strain (Pseudomonas sp) (>107 cell/ml) was the highest of these seven kinds of bacteria. The anammox activities of the seven strains were evaluated. Only E1 strain made ammonium oxidize in anaerobic condition. E1 was a denitrifying bacterium and had some anammox activity.