In microfluidic chips, most micro-channel cross-section shapes are rectangular or triangular in existing chip fabricating technologies, including hot embossing, lithography, etching, injection molding, etc. However, compared with the above micro-channel shapes, a circular shape has advantages in aspects of fluid flow, droplet generation, heat transfer and blood vessel replication. This paper presents a sandwich-like microfluidic chip with circular cross-section micro-channels. The sandwich-like structure includes three layers. The top and bottom layers are PDMS material while the middle layer is composed of micron glass capillaries (used as micro-channels) with circular cross-sections. The glass capillaries are made of borosilicate tube by a glass heating process. Sphere shaped paraffin wax is used as a sacrificial material to form micro-channel junctions. To test the functions of the fabricated micro sandwich-like microfluidic chip, a droplet generating experiment was conducted in the T-junction chip. The droplet size can be controlled in the range of 20–400 μm by varying the water and oil flow rates. This proposed microfluidic chip structure has the advantages of short processing cycle, low cost and small flow resistance.