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In this contribution, we discuss aspects of spectral tuning of molecular chromophores at the atomic level. The basis for the discussion is a series of measurements performed at the electrostatic ion-storage ring ELISA at the University of Aarhus. The experimental technique, which is a type of “action spectroscopy” based on photo dissociation, will briefly be discussed. We will present examples of spectral tuning due to external charges and proton exchange with the hosting medium.