The geographical patterns of plant species richness at the large scale is one of the most pervasive features of life on Earth. This study described this pattern for plants from 18 nature reserves in China subtropical zone and explored the potential explanations behind this pattern. By means of Pearson's correlation analyses, the hidden forces that shape the plant species richness pattern in China subtropical zone were found. The findings showed that plant species richness variation was explained by both energy and water measured as annual potential evapotranspiration and minimum monthly precipitation, respectively. Moreover, climatic heterogeneity in nature reserve habitat explained more variance in plant species richness than topographical heterogeneity. This study emphasizes the importance of assessing the role of climatic heterogeneity in shaping plant species richness pattern.