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  • articleOpen Access

    VSA-TOOL: A TOOL FOR DATA VISUALIZATION IN SEQUENCE ALIGNMENT

    Sequence alignment is a fundamental and important tool for sequence data analysis in molecular biology. Many applications in molecular biology require the detection of a similarity pattern displayed by a number of DNA and protein sequences. Visual front-ends are useful for an intuitive viewing of alignment and help to analyze the structure, functions, and evolution of the DNA and protein. In this paper, we designed and implemented an interactive system for data visualization in DNA and proteins, which can be used in determining a sequence alignment, similarity search of sequence data, and function interference. Experimental results show that a user can easily operate the system after one hour's practice on the proposed system, which provides a clean output, easy identification of similarity and visualization of alignment data.

  • articleNo Access

    USING DIT-FFT ALGORITHM FOR IDENTIFICATION OF PROTEIN CODING REGION IN EUKARYOTIC GENE

    The new research platform on biomedical engineering by Digital Signal Processing (DSP) is playing a vital role in the prediction of protein coding regions (Exons) from genomic sequences with great accuracy. We can determine the protein coding area in DNA sequences with the help of period-3 property. It has been seen that in order to find out the period-3 property, the DFT algorithm is mostly used but in this paper, we have tested FFT algorithm instead of DFT algorithm. DSP is basically concerned with processing numerical sequences. When digital signal processing used in DNA sequences analysis, it requires conversion of base characters sequence to the numerical version. The numerical representation of DNA sequences strongly impacts the biological properties mirrored through the numerical genre. In this work, the proposed technique based on DIT-FFT algorithm has been used to identify the exonic area with the help of integer value representation for transforming the DNA sequences. Digital filters are used to read out period 3 components from the output spectrum and to eliminate the unwanted high frequency noise from DNA sequences. To overcome background noise means to suppress the non-coding regions, i.e., Introns. Proposed algorithm is tested on four nucleotide sequences having single or multiple numbers of exons.