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Let M be a non-abelian semi-direct product of a cyclic group ℤ/n and an elementary abelian p-group A=⊕k(ℤ/p) of order pk, p being a prime and gcd(n,p)=1. Suppose that the knot group G(K) of a knot K in the 3-sphere is represented on M. Then we conjectured (and later proved) that the twisted Alexander polynomial Δγ,K(t) associated to γ:G(K)→M→GL(pk,ℤ) is of the form: ΔK(t)1−tF(tn), where ΔK(t) is the Alexander polynomial of K and F(tn) is an integer polynomial in tn. In this paper, we present a proof of the following. For a 2-bridge knot K(r) in H(p), if n=2 and k=1, then F(t2) is written as f(t)f(t−1), where H(p) is the set of 2-bridge knots whose knot groups map on that of K(1/p) with p odd.