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Object: In this paper, eight male basketball players were tested for physical fitness using the method of sports biology. The content of the physical examination is the isokinetic muscle strength of the shoulder joint. This paper aims to investigate the effect of shoulder isokinetic muscle strength on athletic performance. Methods: This paper uses the principles of sports biology and the isokinetic muscle strength test system to measure the shoulder torque and fatigue index of eight basketball players. At the same time, this paper analyzes the obtained data using the method of mathematical statistics. Results: There were significant differences in the test data of eight male basketball players (P < 0.05). The fatigue of basketball players is mainly in the middle and late stages of sports. At average speed, the fatigue index of the shoulder is the lowest at 180°/s. Conclusion: Basketball players have stronger shoulder extensors than flexors. The balance of strength in the shoulder flexors is greater than the balance in the extensors.