Narrow Results

We study perturbations of a class of analytic two-dimensional autonomous systems with perturbations depending periodically on time; for instance one can imagine a periodically driven or forced system with one degree of freedom. In the first part of the paper, we revisit a problem considered by Chow and Hale on the existence of subharmonic solutions. In the analytic setting, under more general (weaker) conditions on the perturbation, we prove their results on the bifurcation curves dividing the region of non-existence from the region of existence of subharmonic solutions. In particular our results apply also when one has degeneracy to the first order — i.e. when the subharmonic Melnikov function is identically constant. Moreover we can deal as well with the case in which degeneracy persists to arbitrarily high orders, in the sense that suitable generalizations to higher orders of the subharmonic Melnikov function are also identically constant. The bifurcation curves consist of four branches joining continuously at the origin, where each of them can have a singularity (although generically they do not). The branches can form a cusp at the origin: we say in this case that the curves are degenerate as the corresponding tangent lines coincide. The method we use is completely different from that of Chow and Hale, and it is essentially based on the proof of convergence of the perturbation theory. It also allows us to treat the Melnikov theory in degenerate cases in which the subharmonic Melnikov function is either identically vanishing or has a zero which is not simple. This is investigated at length in the second part of the paper. When the subharmonic Melnikov function has a non-simple zero, we consider explicitly the case where there exist subharmonic solutions, which, although not analytic, still admit a convergent fractional series in the perturbation parameter.

Under conditions of stress and environmental fluctuations, how do coupled biochemical systems maintain the balance of metabolic fluxes? Using a model derived from a representative glycolysis example, this work derives sufficient coordination conditions for guaranteeing the balance of metabolic fluxes in the coupled systems subjected to any fluctuations. For diffusion-like coupling, it is explicitly shown that the sufficient conditions are the linear summation of individual uncoupled systems. For this case, coupling strength is not a key factor affecting the balance of metabolic fluxes, although it may affect the emergence of certain dynamic patterns. When the sufficient coordination conditions are satisfied, coupled systems always settle onto stable finite states, independently of the nature (type, period or amplitude) of external signals. When they are not, external signals may force the stable states of coupled systems to lose their coordination, resulting in the coupled systems having no stable finite states which is inconsistent with most normal biological functions. Numerical simulations support the analytical results. The generalization of this result for other coupled nonlinear biochemical systems is discussed. Finally, the maintenance of such biological coordination is discussed in the context of genetic engineering and environmental fluctuations.