The effect of helium on the irradiation damage characteristics and radiation induced segregation at grain boundaries in the welding heat affected zone (HAZ) of Fe-Cr-Mn(W,V) alloy for fusion-fission mixed reactor were investigated by high voltage electron microscopy connected with a high energy ion accelerator. Experimental results showed that the helium enhanced formation of dislocation loop and of void nuclei from initial stage of irradiation, so that void swelling was increased. Furthermore the concentration of solute atoms of Cr, Mn decreased and concentration change of Ni, V, Si, W at grain boundaries were suppressed effectively. The HAZ of Fe-Cr-Mn(W,V) alloy has superior resistance for irradiation damage under existence of helium.