The bifurcation problems of twisted heteroclinic loop with two hyperbolic critical points are studied for the case ρ11>λ11ρ11>λ11, ρ12<λ12ρ12<λ12, ρ11ρ12<λ11λ12ρ11ρ12<λ11λ12, where −ρ1i<0−ρ1i<0 and λ1i>0λ1i>0 are the pair of principal eigenvalues of unperturbed system at the critical point pipi, i=1,2i=1,2. Under the transversal conditions, the authors obtained some results of the existence and the number of 1-homoclinic loop, 1-periodic orbit, double 1-periodic orbit, 2-homoclinic loop and 2-periodic orbit. Moreover, the relative bifurcation surfaces and the existence regions are given, and the corresponding bifurcation graphs are drawn.