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The bio-inspired robotics use functional elements of natures for inspiration. The development of the TB-Horse II prototype is the main target of this work. It is a bio-inspired quadruped robot with biological features in horse of the breed Mangalarga Marchador. In future, the robot can be used to rescue injured people, to carry fragile loads, among others applications. With the study of horse biodynamic, it was possible to propose the TB-Horse II. The gait marcha was implemented and validated using the Virtual Robot Experimentation Platform (V-Rep). Finally, the robot prototype was developed and the experimental validation was realized on a flat ground without obstacles.