[in Journal: Surface Review and Letters] AND [Keyword: Incubation] : Search

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[in Journal: Surface Review and Letters] AND [Keyword: Incubation] (1)	27 Mar 2025	Run
Keyword: Sensitizers (3)	27 Mar 2025	Run
[in Journal: Journal of Enterprising Culture] AND [Keyword: Competence] (1)	27 Mar 2025	Run
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articleNo Access
VISUALIZATION EX SITU OF SINGLE DNA MOLECULES INCUBATION: A FIRST STEP FOR QUANTITATIVE ANALYSIS ON MULTI-SITE DEGRADATION AND ENZYMATIC KINETICS
- XINYAN WANG,
- HAIJUN YANG,
- HUABIN WANG,
- PENG WANG, and
- HAI LI
Surface Review and Letters01 Feb 2009
Preview Abstract
Herein, we showed a different approach to directly single-molecule level visualization of the degradation of DNA in vitro tests using DNase I incubation based on high-resolution AFM imaging ex situ with fine relocation nanotechnology. A method of nanomanipulation termed as "modified dynamic molecular combing" (MDMC) was used to pattern DNA samples for further degradation and enzymatic kinetics. This strategy is potentially able to quantitatively address the mechanical-induced kinetic profiles of multi-site degradation of individual DNA molecules with very stable tension and strong immobilization on a surface and discover the mechanochemistry.

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