This work aimed to evaluate the potential of biosurfactant produced by Rhdotorula glutinis using industrial wastes as substrates. The production was made in flasks (250 ml) containing 100 ml of means of production according to an experimental kind CCRD, varying the concentration of cassava wastewater, waste corn steep liquor and ice cream. Later, he was selected the best condition to make the factorial design optimization. Production was transferred to the reactor with a capacity of dual-shirt 2L under the same conditions. Were inoculated 5% of the pre-inoculum (Sabouraud Dextrose Broth), fermentation took place for 72 hours, 150 rpm at 28°C. Were determined the surface tension and interfacial fluid cell-free metabolic, obtaining surface tension de28, 2 mN/m interfacial 5.1 mN/m. Has been demonstrated effective stability of biosurfactant at different pH’s, temperatures and salinity determined by tensão. Subsequently was determined emulsification index of stability. The results obtained for biosurfactant production by R. glutinis using industrial waste is an alternative that comes to the economy and contribute to the preservation of the environment