Narrow Results

Molecular biology combined with in silico machine learning and deep learning has facilitated the broad application of gene expression profiles for gene function prediction, optimal crop breeding, disease-related gene discovery, and drug screening. Although the acquisition cost of genome-wide expression profiles has been steadily declining, the requirement generates a compendium of expression profiles using thousands of samples remains high. The Library of Integrated Network-Based Cellular Signatures (LINCS) program used approximately 1000 landmark genes to predict the expression of the remaining target genes by linear regression; however, this approach ignored the nonlinear features influencing gene expression relationships, limiting the accuracy of the experimental results. We herein propose a gene expression prediction model, L-GEPM, based on long short-term memory (LSTM) neural networks, which captures the nonlinear features affecting gene expression and uses learned features to predict the target genes. By comparing and analyzing experimental errors and fitting the effects of different prediction models, the LSTM neural network-based model, L-GEPM, can achieve low error and a superior fitting effect.

The succinylation modification of protein participates in the regulation of a variety of cellular processes. Identification of modified substrates with precise sites is the basis for understanding the molecular mechanism and regulation of succinylation. In this work, we picked and chose five superior feature codes: CKSAAP, ACF, BLOSUM62, AAindex, and one-hot, according to their performance in the problem of succinylation sites prediction. Then, LSTM network and CNN were used to construct four models: LSTM-CNN, CNN-LSTM, LSTM, and CNN. The five selected features were, respectively, input into each of these four models for training to compare the four models. Based on the performance of each model, the optimal model among them was chosen to construct a hybrid model DeepSucc that was composed of five sub-modules for integrating heterogeneous information. Under the 10-fold cross-validation, the hybrid model DeepSucc achieves 86.26% accuracy, 84.94% specificity, 87.57% sensitivity, 0.9406 AUC, and 0.7254 MCC. When compared with other prediction tools using an independent test set, DeepSucc outperformed them in sensitivity and MCC. The datasets and source codes can be accessed at https://github.com/1835174863zd/DeepSucc.