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Purpose: This study aimed to systematically examine the influence of various muscle and experimental conditions on Myoton recordings. Methods: A cross-sectional, observational design was used to examine muscle conditions and experimental factors (different recording sites, muscle length, level of contraction and prior physical activity) that may influence reproducibility of Myoton recordings for biceps brachii (BB) and rectus femoris (RF). Fifty-three healthy adults (26 young, 27 older) aged 18–90 years were studied. Muscle stiffness, tone and elasticity were measured using the MyotonPRO device. Results: Statistically significant differences in Myoton parameters were found for aspects of all four muscle and experimental conditions compared with the control condition ($p<0.05$). However, clinically relevant differences in tone, stiffness and elasticity were only found for contracted compared to resting muscle, with changes being greater than the minimal detectable change. Elasticity was not affected by prior activity. Conclusions: The conditions studied significantly altered Myoton parameters of BB and RF in healthy adults, but only changes in parameters during muscle contraction were clinically relevant. These findings provide evidence to support the need to consider muscle condition and experimental factors for improving the robustness of test protocols for assessing muscle mechanical properties using the MyotonPRO device.

The purpose of this study is to investigate how the pain and physiological characteristics of the trapezius change when the Winback therapy is applied to the trapezius in patients with work-related musculoskeletal disorders (WMSDs). This study was conducted on 30 healthy adults living in Jeju Island. After applying the Winback 3SE high-frequency equipment developed in France, the muscle tone, muscle stiffness, pressure pain threshold, and visual analog scale (VAS) of the trapezius were measured. After the intervention, muscle tone decreased from 16.15 ± 2.02 to 14.22 ± 1.83, muscle stiffness decreased from 285.81 ± 58.26 to 245.83 ± 51.62, pressure pain threshold increased from 62.58 ± 23.28 to 79.38 ± 28.27, and VAS decreased from 3.73 ± 0.73 to 1.01 ± 0.02. These improvements were statistically significant (p < 0.05), and the muscle tone, muscle stiffness, pressure pain threshold improved in the experimental group compared to the control group. The Winback therapy was effective in reducing muscle tone, muscle stiffness, and pain of trapezius in WMSDs.