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To determine the susceptibilities of Mycoplasma homonis (M. hominis) to Chinese medicinal herbs in vitro, 30 clinical strains of M. hominis were isolated and identified from the clinical specimen. The susceptibilities of M. hominis to 19 herbs were determined by serial dilution methods in vitro. The results showed that M. hominis was susceptible to Radix Isatidis, Radix Angelicae Dahuricae, Cortex Phellodendri, Radix et Rhizoma Rhei, Fructus Kochiae and Herba Houttuyniae. These findings laid a foundation in treating M. hominis infection with Chinese herbs.