Let the self-similar measure μM,D be generated by an expanding real matrix M=ρ−1I∈M2(R) and a digit set D={(0,0)t,(1,0)t,(0,1)t,(−1,−1)t} in space R2. In this paper, we only consider ρ>0 and the case ρ<0 is similar. We show that there exists an infinite orthogonal set of exponential functions in L2(μM,D) if and only if ρ=(q/(2p))1r for some p,q,r∈N with gcd(2p,q)=1. Furthermore, for the cases that L2(μM,D) does not admit any infinite orthogonal set of exponential functions, the exact cardinality of orthogonal exponential functions in L2(μM,D) is given.