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    Chapter 6: The Context – Form Informing Function

    This chapter reveals the depth of the transformational nature inherent in the Mereon Matrix showing how clusters and singles of polyhedra, 33 unified forms, scale to infinity, internally and externally. This chapter suggests significant physical, psychological and sociological implications inherent in the structure and dynamics of the Matrix’s outermost polyhedron. Referred to as the Context, this geosphere iterates between 120 and 180 faces as it opens and closes, expands and contracts. Image intense, it is revealed how localised environmental energy is required, received and processed to eventually produce new systems and/or product that expands the global environment.

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    Study on selection and transfer of antimicrobial resistant Escherichia coli from broiler breeders to their progeny

    Escherichia coli is a normal inhabitant of the intestinal tract of chickens, but when an imbalance in bacterial flora of the intestinal tract occurs, E. coli may over grow and cause extraintestinal infections. The aim of this study was to evaluate the selection and transfer of E. coli clones and their antimicrobial resistant characters from broiler breeders to their progeny in absence or presence of an ongoing E. coli infection. Environmental samples and different organs of humanly euthanized broiler breeder were collected from group R1 and its sick progeny P1 (characterised by omphalitis symptoms) and from group R2 and its healthy progeny (P2). All samples were tested for the presence of E. coli and the isolates obtained were typed using XbaI Pulsed Field Gel Electrophoresis and EcoRI automated ribotyping. The 172 E.coli isolates were molecular discriminated into 90 XbaI PFGE types and 63 ribotypes. Five E. coli isolates from breeder R1 and four from breeder R2 shared the same PFGE and/or ribotype with E. coli isolates from progeny P1 and P2. Interestingly E. coli strains isolated both in breeders and their progeny showed different antibiotic multiresistance profiles reinforcing the idea of a possible horizontal transfer of antibiotic resistance determinant genes to and from these isolates.