We describe a simple and effective approach to the introduction of a functional group into polymer nanofilms on the solid surface using reactive Langmuir–Blodgett films. N-Dodecylacrylamide copolymers containing a terminal amino group in the side chains as a reactive moiety form a stable monolayer on a water surface, and the monolayer was transferred onto a solid support with a transfer ratio of unity. The LB films were characterized by various spectroscopic methods. The reactivity of the terminal amino group incorporated in the LB films was investigated in detail using fluorescein isothiocyanate (FITC) as a fluorescent probe. The chemical reaction between the amino group in the LB films and FITC in the bulk solution was completed within approximately 30 min and the chemical bond formation was confirmed by FT-IR spectroscopy. Furthermore, the ability as a pH sensor was observed with fluorescent microscopy.
