This paper aims at examining the enterprises characteristic, business performance, and obstacles in doing business for food and agribusiness firms across rural and urban areas, based on World Bank’s Enterprises Survey data of 699 respondents. Chi-square test shows statistically significance difference in firms’ characteristics across the rural and urban regions. ANOVA reveals a significant difference in business performance. Similarly, perception about obstacles in doing business varies across the urban and rural firms. This study can be helpful for agribusiness managers and government policy makers in promoting region-based entrepreneurship in a focused manner.