The tissue characterization of coronary plaque is essential for a diagnosis of acute coronary syndromes. The radio frequency (RF) signals obtained by the intravascular ultrasound (IVUS) method are the representative data for tissue characterization of coronary plaque. RF signals are converted to luminosity values, and the cross sectional images of coronary artery can be observed, which is to evaluate the condition of acute coronary syndromes in real time. The images however are obscured by the attenuation of the ultrasound depending on the distance from the ultrasonic probe and by the ultrasound scattering due to red blood cells. Intravascular tissue characterization based on the analysis of RF signals, as an attempt to evaluate tissue structure quantitatively, have been proposed. Representative conventional methods use the analysis of the frequency characteristics of RF signals. However, the computing cost is high and the settings of the parameters are complicated and dificult. In this paper, tissue characterization of coronary plaque by subspace method is newly proposed. The proposed method, not only considers the neighborhood information around the target tissue, but also realizes a low computing cost and has easy parameter settings. The effectiveness of the proposed method has been verified by the application to the actual tissue characterization problem of the human coronary plaque.