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This manual is designed to serve as a practical guide to primary human cell culture, which is integral in both academic and industrial biotechnology research. As in the first edition, the content of the manual is not exhaustive, but rather contains selected protocols for specific cell types from major tissue groupings in the body. This improved second edition also includes a new section on stem cells and additional material on transfection. It should serve as a foundation for individual researchers to experiment, explore, and establish niche protocols for their specific needs. With its compact physical format that makes it portable and flexible for usage in a laboratory setting, the manual will be a useful guide for all beginners in primary human cell culture work.
Sample Chapter(s)
Foreword (47 KB)
Chapter 1: Principles of Biosafety (1,211 KB)
Contents:
- Principles of Biosafety
- Introduction to the Cell Culture Laboratory
- Protocols for Human Cell Culture
- Reagents and Techniques for Cell Culture
- Techniques for Manipulating Cell Cultures and Studying Cellular Behaviour
- Troubleshooting
Readership: Graduate students and scientists embarking on cell culture; biologists; medical practitioners; laboratory technicians; junior college and polytechnic students.
