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The radicals of ·OH and ·H are generated as cavities collapse. The hydroxyl radical, having strong oxidizing property and depending on the cavitation intensity, can react with refractory organic compound molecule. Using the choking cavitator as a cavitation generator, the influences of hydroxyl radical production on the initial concentration of methylene blue (MB), equivalent diameter of back-pressure holes and reaction time were analyzed with a visible spectrophotometer. The experimental results showed that the highest hydroxyl radical was acquired when the equivalent diameter of back-pressure holes was 5.4mm and the initial concentration of MB was 12mg/L.