The quality of 2-year-old Dendrobium officinale Kimura et Migo from four different latitudes Weifang City, Shandong Province (36°78'N, 119°02'E), Wujiang City, Jiangsu Province (31°08'N, 120°66'E), Wenzhou City, Zhejiang Province (28°27'N, 121°05'E) and Menghai City, Yunnan Province (21°59'N, 100°24'E) was analyzed in order to understand the effect of latitude, temperature, humidity, light intensity on its quality. It has important theoretical and practical significance to optimize its cultivation technology and improve its active ingredient content. The results showed that the ecological factors had effects on the quality of 2-year-old D. officinale. The quality of 2-year-old D. officinale in Weifang City, Shandong Province equals to or is higher than that in source regions. D. officinale can be widely planted in high latitude regions such as Weifang City, Shandong Province. The ecological factors for growth D. officinale in high latitude regions are as following: temperature three basis points are 4.7-17.2-32.3°C, mean temperature difference is 4.7-20.5°C, effective accumulated temperature is 1831°C, mean humidity is 69.7-89.5% and mean light intensity is 1.9-19.5 Klux.