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In vitro incubation of tissues; in particular, skeletal muscles from rodents, is a widely-used experimental method in diabetes research. This experimental method has previously been validated, both experimentally and theoretically. However, much of the method's experimental data remains unclear, including the high-rate of lactate production and the lack of an observable increase in glycogen content, within a given time. The predominant hypothesis explaining the high-rate of lactate production is that this phenomenon is dependent on a mechanism in glycolysis that works as a safety valve, producing lactate when glucose uptake is super-physiological. Another hypothesis is that existing anoxia forces more ATP to be produced from glycolysis, leading to an increased lactate concentration. The lack of an observable increase in glycogen content is assumed to be dependent on limitations in sensitivity of the measuring method used. We derived a mathematical model to investigate which of these hypotheses is most likely to be correct. Using our model, data analysis indicates that the in vitro incubated muscle specimens, most likely are sensing the presence of existing anoxia, rather than an overflow in glycolysis. The anoxic milieu causes the high lactate production. The model also predicts an increased glycogenolysis. After mathematical analyses, an estimation of the glycogen concentration could be made with a reduced model. In conclusion, central anoxia is likely to cause spatial differences in glycogen concentrations throughout the entire muscle. Thus, data regarding total glycogen levels in the incubated muscle do not accurately represent the entire organ. The presented model allows for an estimation of total glycogen, despite spatial differences present in the muscle specimen.

Entrepreneurs are a product of their social environment. The manner by which they perceive opportunities; access or process information; and make decisions is, influenced by both social interaction, and their social background. Using insights from Socially Situated Cognition (SSC) theory, that posits one’s social environment can have a normative or informative effect on decision-making process we consider proximal social factors influencing the decision-making processes of student entrepreneurs. We propose that entrepreneurial education, networking, and incubation spaces provide direct information to students to aid entrepreneurial decision-making, and indirect informational cues that are situational, synergistic and omnipresent. Noting the multi-faceted and dynamic nature of the entrepreneurial journey of the student, we explore the potential effect of each of these factors on the student decision-making process. We discuss the implications of this inquiry from a researcher and educator perspective, and note the current challenges faced by student entrepreneurs in a socially distanced educational and entrepreneurial context. It is envisaged that this paper will serve as the basis for further thought and empiricism.

Herein, we showed a different approach to directly single-molecule level visualization of the degradation of DNA in vitro tests using DNase I incubation based on high-resolution AFM imaging ex situ with fine relocation nanotechnology. A method of nanomanipulation termed as "modified dynamic molecular combing" (MDMC) was used to pattern DNA samples for further degradation and enzymatic kinetics. This strategy is potentially able to quantitatively address the mechanical-induced kinetic profiles of multi-site degradation of individual DNA molecules with very stable tension and strong immobilization on a surface and discover the mechanochemistry.

The article is about Exploit Technologies Pte Ltd that is set up by A*STAR to handle the management and commercialization of the researchers' IP.

The Biotechnology Incubation Center of Academia Sinica: A Gateway to Innovative Taiwan.

Cancer Targeting Nanomedicine: An Opportunity for Drug Development in Asia with Emphasis on Taiwan.

Recombinant Lipoproteins for the Development of Novel Subunit Vaccines.

Discovery of Therapeutic Human Monoclonal Antibodies through B-cell Immortalization Technology.

This paper highlights the relevance of innovation in small firms based on university business incubators in Portugal. Ever since Schumpeter indicated innovation to be the main economic driver of economic development and a critical factor in the development and performance of companies, a number of authors have discussed this phenomenon but there is still no consensus on how it operates. Through statistical analysis, the study focuses on a sample of 243 Portuguese companies based on incubators linked to or promoted by universities. It was concluded that product innovation is most significant in an incubation environment, and also that the origin of businesses and the development of R&D activities determine the type of innovation generated. However, most of the companies surveyed are not engaged in R&D activities. We propose some policy measures and lessons for management.