This experiment was mainly prepared for investigating mechanical properties and characterization of the chitosan-corn starch-methyl cellulose 3-layer film, in comparison to the single chitosan, starch and methyl cellulose film respectively. Through single factor experiment, we obtained the film deposition conditions. The structural characterization and mechanical properties were observed and measured by the scanning electron microscope (SEM) and universal tensile machine. Experiments indicate the prepared conditions of 3-layer film are 50g of concentration 2% (w/w) chitosan solution, 10g corn starch solution (5% w/v) and methylcellulose at a concentration of 2% (w/v) for 10g. The results showed that all plasticized films surfaces exhibited homogeneous structures, and the three-layer structure of the chitosan-corn starch-methyl cellulose 3-layers film could be observed clearly. However, the mechanical properties of the three-layer film are not significantly increased compared with other controls groups. When will the layer stack destroy the original network structure still needs further validation.