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We studied the effects of aluminum on macro- and microelement concentrations in white roots of Chamaecyparis obtuse and Quercus serrata Thunb. through analysis by Particle Induced X-ray Emission (PIXE). One-year-old seedlings were exposed to various concentrations of Al for nine weeks. In both species, the Al and P concentrations increased as the Al concentration in the nutrient solution increased, but the concentrations of the divalent cations Mg, Zn, and Mn decreased in both species.